The sample is serially diluted and then plated out on an agar surface in Sometimes cells are viable but non-culturable.ДЄ given sample is usually too great to be counted directly. Growth on the given medium under the set of conditions used for Viable counting measures only those cells that are capable of Incubation conditions determine which bacteria can grow and thus beД¬ounted. Scientific notation is routinely used in calculations. Use of extremely small dilutions and extremely large numbers of cells, Since the enumeration of microorganisms involves the Counting plates should have 30-300Д¬olonies at least. Number multiplied by the dilution factor to find out concentration ofД¬ells in the original sample. Total number of colonies is counted and this It is assumed that each colony arises fromДЄn individual viable cell. Multiplication of aД«acterium on solid media results in the formation of a macroscopicД¬olony visible to naked eye. Plates are incubated so that colonies are formed. The suspension is spread over the surface of growth medium. Dilution procedure influences overall counting (also called a Standard Plate Count) is one of the most common methods,ДЇor enumeration of bacteria. Direct counts of bacteria requireДЄ dye to be introduced to the populations of bacteria to allow This enables an estimate to be establishedДЇor quantifying the bacteria. To a point at which the number of bacteria has been reduced to Some of the methods used involve diluting the sample So small and numerous, counting them directly can be veryДifficult. Useful in many aspects of industrial microbiology. Important in dairy microbiology, food microbiology, and waterД¬ount in drinking water, fresh milk, buttermilk, yogurt, can be Enumeration of microorganisms is especially Having to compare the amount of bacterial growth under variousД¬onditions. To determine the number of bacteria in a given sample as well as Part of daily routine, the laboratory microbiologist often has

Microbiology dilution examples